C5aR1/β-Arrestin/CHO
CBP71384
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| I. Background | |
| 補(bǔ)體 C5a 是由補(bǔ)體系統(tǒng)經(jīng) C5 轉(zhuǎn)化酶裂解 C5 產(chǎn)生的強(qiáng)效炎癥介質(zhì)，作為核心過敏毒素 通過結(jié)合受體 C5aR1/C5aR2 驅(qū)動(dòng)中性粒細(xì)胞趨化、氧化爆發(fā)及炎性因子（IL-6/TNF-α）釋 放，在膿毒癥中誘發(fā)“細(xì)胞因子風(fēng)暴”，在自身免疫?。ㄈ珙愶L(fēng)濕關(guān)節(jié)炎）中促進(jìn)組織侵 蝕，在腫瘤微環(huán)境募集髓系抑制細(xì)胞（MDSC）介導(dǎo)免疫逃逸。 C5aR1（CD88）是補(bǔ)體過敏毒素 C5a 的高親和力 GPCR，主要表達(dá)于髓系細(xì)胞（中性 粒細(xì)胞、單核細(xì)胞、肥大細(xì)胞），通過激活 Gi/Gq 蛋白-PI3K/MAPK 通路驅(qū)動(dòng)炎癥級(jí)聯(lián)反 應(yīng)。 |
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| II. Description | |
C5aR1 β-Arrestin CHO 報(bào)告基因藥靶模型很好的模擬了體內(nèi) C5aR1/β-Arrestin 的信號(hào)轉(zhuǎn)導(dǎo)過程，原理見下圖所示。 Figure 1. C5aR1 β-Arrestin CHO 細(xì)胞模型原理圖 |
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| III. Introduction | |
| Host Cell: |
CHO |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5 μg/ml Puromycin+5 μg/ml Blasticidin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for C5aR1 |
| Transducer: | β-Arrestin |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
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Figure 3. Dose Response of Human C5a in C5aR1 β-Arrestin CHO (C8). |
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Figure 4. Inhibition of hC5a-induced Beta-Arrestin Recruitment by hC5a Neutralization Ab in C5aR1 Beta-Arrestin CHO(C8). |
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