CCR8/CHO
CBP71364
| I. Background | |
|
趨化因子(C-C motif)受體 8 (CCR8)是一種主要表達于調(diào)節(jié)性 T 細胞(Treg)上的趨化因子 受體，屬于 G 蛋白偶聯(lián)受體。CCR8 的配體有 CCL1、CCL8、CCL16 和 CCL18。 |
|
| II. Description | |
|
cAMP 在 G 蛋白偶聯(lián)受體(GPCR)信號轉(zhuǎn)導(dǎo)中是一種關(guān)鍵的第二信使。當(dāng)配體結(jié)合到 GPCR，構(gòu)象發(fā)生變化，激活受體接著激活 G 蛋白，Gi 的激活導(dǎo)致腺苷酸環(huán)化酶調(diào)控 cAMP 的下調(diào)。CCR8 藥靶模型很好的模擬了體內(nèi) CCR8 的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
|
|
| III. Introduction | |
| Host Cell: |
CHO |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5μg/ml puromycin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for CCR8 |
| Transducer: | Gi |
| IV. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| V. Representative Data | |
|
Figure 2. Recombinant CCR8/CHO constitutively expressing CCR8. |
|
|
Figure 3. HTRF cAMP Assay with CCR8 CHO(C36). |
|
