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hERG/HEK293

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I. Background
ERG (ETS Transcription Factor ERG) is a Protein Coding gene. Diseases associated with ERG include Ewing Sarcoma and Lymphatic Malformation 14.
 
II. Introduction
Host Cell: HEK293
Expressed gene: hERG
Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium: 90% FBS+10% DMSO
Culture Medium:

DMEM + 10% FBS+100 μg/ml Zeocin

Storage: Liquid nitrogen
 
III. Description of Host Cell Line
Organism: Homo sapiens, human
Tissue: Embryonic kidney
Disease: Normal
Morphology: Epithelial
Growth Properties: Adherent
 
IV. Representative Data


Figure 1. Inhibition of hERG current by Cisapride.



Figure 2. raw current traces responding to vehicle and six doses of compound.

 

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